Alkaline agar

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Specification

(Nutrient medium for the isolation and cultivation of Vibrio cholerae dry)

Feature:

The nutrient medium is used for the cultivation of Vibrio cholerae and isolating it from the infected material.

Is a fine powder from light yellow to light brown, hygroscopic.

Ingredients:

Pancreatic hydrolyzate of fish meal, peptone, dry fermentation, yeast extract, glucose, sodium phosphate dibasic, sodium chloride, sodium metabisulfite, sodium carbonate agar.

Preparation:

Drug in an amount necessary for the preparation of a series of specific nutrient medium in a stirred 1 liter of distilled water, heated until complete melting of the agar, filtered through cotton-gauze filter, dispensed in vials and sterilized by autoclaving for 20 minutes at a temperature
(121 ± 1) ° C.

Ready nutrient medium transparent yellow. Light opalescence is permitted.

pH 8,0 ± 0,2

Prepared medium can be stored for 10-14 days at 2-8 ° C in a dark place.