The nutrient medium for the isolation of Shigella and Salmonella dry
Culture medium intended for the isolation of Salmonella and Shigella test material (faeces, urine, etc.), And differentiating them from other enterobacteria.
Is a fine, hygroscopic, light-sensitive powder of light yellow color.
Pancreatic hydrolyzate of fish meal, dry with thiosulfate and sodium citrate, lactose, yeast extract, dry purified bile, sodium phosphate dibasic, sodium chloride, neutral red, brilliant green, crystal iodine agar.
Drug in an amount necessary for the preparation of a series of specific nutrient medium, thoroughly stirred in 1 liter of distilled water, boiled for 3 minutes, stirring occasionally until a complete melting of the agar. Cooled to a temperature of 40-45 ° C, poured into petri dishes layer 5-6 mm and left to solidify. Before inoculation the medium cup desktop dried with the lids open during 90-100 minutes at a temperature of 18-25 ° C.
Finished plates are clear brownish-red color.
pH 7,0 ± 0,2
The finished medium poured into a Petri dish, can be used within 2 days after its preparation when stored in a dark place at a temperature of 18-25 ° C.