The nutrient medium for the accumulation of dry Listeria

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Specification

Feature:

Environment is intended for accumulation of pathological material Listeria adding the selective agent in order to suppress the accompanying flora.

Ingredients:

Nutrient broth dry fodder yeast extract, glucose, disodium phosphate, sodium chloride.

Preparation:

23 grams of the drug mix in 1 liter of distilled water, bring to a boil, boil for 2 minutes. Before sterilization make nalidixic acid (0.5 g nalidiksoyvoy acid dissolved in 0.5 ml of 1% NaOH by adding 4.5 ml of distilled water) in 1 L of medium.

Autoclaved at 115ºS for 30 min. Pour into sterile tubes 4.5 ml.