Environment is intended for accumulation of pathological material Listeria adding the selective agent in order to suppress the accompanying flora.
Nutrient broth dry fodder yeast extract, glucose, disodium phosphate, sodium chloride.
23 grams of the drug mix in 1 liter of distilled water, bring to a boil, boil for 2 minutes. Before sterilization make nalidixic acid (0.5 g nalidiksoyvoy acid dissolved in 0.5 ml of 1% NaOH by adding 4.5 ml of distilled water) in 1 L of medium.
Autoclaved at 115ºS for 30 min. Pour into sterile tubes 4.5 ml.