The nutrient medium for the isolation and cultivation of Listeria dry

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Specification

Feature:

Environment is intended for the isolation and cultivation of the infected material Listeria when added selective agents.

Ingredients:

Nutrient agar dry fodder yeast extract, dextrose, thiamin bromide, disodium phosphate.

Preparation:

43.5 grams of the drug mix in 1 liter of distilled water, bring to a boil, boil for 2 minutes. Before sterilization make nalidixic acid (0.04 g nalidiks.kisloty dissolved in 0.1 ml of 1% NaOH by adding 1.5 ml of distilled water) and add to 1 liter of medium. Autoclaving at a temperature of 115º C for 30 min. Before use, sterile environment cooled to a temperature (47,5+ 2,5) º C and pour into sterile Petri dishes.