(Nutrient medium to determine the susceptibility of microorganisms to antibiotics dry)
Culture medium intended for determination of antibiotic susceptibility of microorganisms isolated from pathological material patients "by drives."
Is a fine hygroscopic yellow powder
Pancreatic hydrolyzate of fish meal, sodium chloride, starch, sodium phosphate dibasic, agar.
The drug in the amount indicated on the label for the preparation of a series of specific nutrient medium in a stirred 1 liter of distilled water, boiled for 1-2 min until complete melting of the agar, filtered through cotton-gauze filter and dispensed into containers and sterilized at 121 ° C for 15 min. The medium is cooled to a temperature of 45-50 ° C and poured into sterile Petri dishes layer 4-6 mm. After solidification of the medium in the plates are dried for 50-60 minutes at a temperature (37 ± 1) ° C.
Prepared medium transparent yellowish light opalescence is permitted.
pH 7,4 ± 0,2