Nutrient medium Eijkman with glucose

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Specification

(Nutrient medium for the detection of E. coli and coliform bacteria on the basis of dry fermentation of glucose)

TU 9398-088-78095326-2008

Feature:

Culture medium intended for the detection of E. coli and coliform bacteria based on the fermentation of glucose with sanitary bacteriological examination of water and tap water from other sources.

Is a fine hygroscopic powder of light yellow color.

Ingredients:

Pancreatic digest fish meal, peptone dry enzyme, glucose, sodium chloride, sodium carbonate, bromothymol blue.

Preparation:

To investigate the use of a conventional medium and conc.

To prepare a sample of the usual concentration of the medium in the amount indicated on the label for the preparation of a series of specific nutrient medium in a stirred 1 liter of distilled water, boiled for 1-2 minutes, filtered through cotton-gauze filter, poured into 10 ml containers sterilized with floats and autoclaving at a temperature of 110 ° C for 15 min.

Prepared medium transparent green.

To prepare a sample of the concentrated medium increased by 10 times, boiled for 1-2 minutes, filtered through cotton-gauze filter, is poured into the container 1, with the floats 10 ml, sterilized by autoclaving at 110 ° C for 15 min.

Prepared medium brick-green color. Possible opalescence.

pH 7,0 ± 0,2

Prepared medium can be used within 10 days when stored at 2-8 ° C.