Nutrient medium Eijkman with lactose

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Specification

(Nutrient medium for the detection of E. coli and coliform bacteria on the basis of lactose fermentation dry)

TU 9398-089-78095326-2008

Feature:

Culture medium intended for the detection of E. coli and coliform bacteria based on lactose fermentation in sanitary and bacteriological examination of water and the tap water from other sources.

Is a fine hygroscopic powder of light yellow color.

Ingredients:

Pancreatic digest fish meal, peptone dry enzyme, lactose, sodium chloride, sodium carbonate, bromothymol blue

Preparation:

To investigate the use of a conventional medium and conc.

To prepare the medium normal concentration of 20 g, is stirred into 1 liter of distilled water, boiled for 1-2 minutes, filtered through cotton-gauze filter, poured into 10 mL capacity with floats and sterilized by autoclaving at 110 ° C for 15 min.

Prepared medium transparent green.

To prepare a sample of the concentrated medium increased by 10 times, boiled for 1-2 minutes, filtered through cotton-gauze filter, is poured into the container 1, with the floats 10 ml, sterilized by autoclaving at 110 ° C for 15 min.

Prepared medium brick-green color. Possible opalescence.

pH 7,0 ± 0,2

Prepared medium can be used within 10 days when stored at 2-8 ° C.