Nutrient medium Hiss

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Specification

(Nutrient medium for the identification of Enterobacteriaceae dry)

TU 9398-049-78095326-2008

Reg. Number FSR 2008 /

Feature:

Hiss-medium RM used for identification of Enterobacteriaceae fermentation of the dough from carbohydrates (lactose, glucose, sucrose, maltose) or a polyhydric alcohol (mannitol).

Is a fine hygroscopic powder of light yellow color.

Hiss-medium RM is produced in the form of five separate preparations, in each of which the composition enters or carbohydrate (lactose, glucose, sucrose, maltose) or a sugar alcohol (mannitol).

Ingredients:

Pancreatic hydrolyzate of fish meal, sodium chloride, sodium phosphate dibasic, bromothymol blue, agar, one of carbohydrates: lactose, glucose, sucrose, maltose or a polyalcohol - mannitol.

Preparation:

Drug in an amount necessary for the preparation of a series of specific nutrient medium in a stirred 1 liter of distilled water, boiled for 3 min to completely melt the agar, filtered through cotton-gauze filter, dispensed in 4 ml sterile tubes, sterilized by autoclaving at 112 ° C. for 20 min.

Prepared medium blue-green color.

pH 7,4 ± 0,2

Prepared medium can be used for 10 days if stored it at 2-8 ° C.