Nutrient medium Levin

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(Nutrient medium with eosin-methylene blue dry)

TU 9398-054-78095326-2008

Reg. Number FSR2008 / 03063


Culture medium intended for isolation and differentiation of pathogenic and conditionally pathogenic enterobacteria, as well as for the isolation of staphylococci.

Is a fine, hygroscopic, light-sensitive powder pale lilac color.


Pancreatic hydrolyzate of fish meal, yeast extract, lactose, sodium phosphate dibasic, sodium chloride, N-Eosin methylene blue agar.


Drug in an amount necessary for the preparation of a series of specific nutrient medium in a stirred 1 liter of distilled water, boiled for 3 minutes, filtered through cotton-gauze filter and sterilized by autoclaving at 110 ° C for 20 min. Sterile medium is cooled to a temperature of 45-50 ° C, poured into a sterile petri dish layer 5-6 mm. After solidification of the plates dried at medium temperature (37 ± 1) ° C for 40-60 min.

Prepared medium in petri dishes clear, from light purple to reddish-brown.

pH 7,2 ± 0,2

Sterile medium can be used within 3 days of storage at condition of its temperature
2-8 C.