Nutrient medium Olkenitsky

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A breeding ground for identification of Enterobacteriaceae Dry (Iron-glucose-lactose agar)

Reg. VFS number 42-416 BC-93


Wednesday is intended for generic identification of enterobacteria by tests: utilization of urea, hydrogen sulfide, fermentation of carbohydrate - lactose, glucose.


Pancreatic hydrolyzate of fish meal with sodium thiosulfate, lactose, glucose, sodium phosphate dibasic, potassium dihydrogen phosphate, sodium chloride, iron oxide citrate, phenol red, sodium sulfite, urea agar.


Drug in an amount necessary for the preparation of the series of culture medium again stirred in 1 liter of distilled water and sterilized by autoclaving at 110 ° C for 15 min. After sterilization, the cooled medium to a temperature 40-50oS poured into 7 ml sterile tubes and Mow, leaving a column height of 25-30 mm.

Prepared medium is red.

Sterile medium can be used for two weeks when it is stored at a temperature or 2-8oS for 3 days at a storage temperature 18-25oS (vials should be stored in a dark place).

pH 7,1 ± 0,2.