Nutrient medium OTDM

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Nutrient medium for the detection of toxigenic diphtheria Germs Dry (OTDM)

Reg. FS number 42-3519-98


Environment is intended to determine the microbial toxigenicity diphtheria precipitation method in agar.


Autolysate sprat, sodium phosphate dibasic, agar, soda ash.


A batch of the drug specified on the label, stir in 1 liter of distilled water, boil for 3-5 minutes until completely melted agar. Pour into sterile 17 ml tubes by aseptically and sterilized by autoclaving for 30 minutes at 110 ° C. The completed medium in the tubes can be stored at 6-8 ° C for 10 days. Before use, the medium in a water bath to melt and, after cooling to 40-50 ° C in a test tube add 3 ml of normal horse serum, previously tested for sterility. Stir the contents and pour into sterile Petri plates with a diameter of 10 ml.